Software
For running Mayu install a perl
interpreter and run the software from the unpacked zip file on a
command line. See the manual,
pdf for more details.
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Mayu source code on GitHub
Mayu package v1.08
- manual
- software package
- example data set
- perl script to create target
decoy databases
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Features
- provides false discovery rates not
only on peptide-spectrum match level (mFDR) but also
on protein identification (protFDR) and peptide
identification (pepFDR) level
- false discovery rates for single hits (single
peptide-spectrum match protein identifications) and all but single hits
(protein identifications with more than one peptide-spectrum match)
- analysis in dependence of the amount of data
- R plots
- provides tables for each input file that relate
the discriminant score (e.g. PeptideProphet, xcorr, mascot ion
score,...) to a peptide-spectrum match false discovery rate
Example Result Plots
perl Mayu.pl -B example.csv -C tardecdb.fa -H 41 -N 4 -O 15 -v -PmFDR -runR
- graphical characterization of the input search results: discriminant score vs. peptide-spectrum match false discovery rate ds_vs_mFDR.pdf
- a number of plots helping the user in selecting the appropriate filtering for the desired quality main.pdf
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